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ma104 cells  (ATCC)


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    ATCC ma104 cells
    Ma104 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ma104 cells/product/ATCC
    Average 96 stars, based on 373 article reviews
    ma104 cells - by Bioz Stars, 2026-04
    96/100 stars

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    Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of <t>MA104,</t> Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).
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    Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

    Journal: Journal of Virology

    Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

    doi: 10.1128/jvi.01883-25

    Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

    Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

    Techniques: CCK-8 Assay, Inhibition

    NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

    Journal: Journal of Virology

    Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

    doi: 10.1128/jvi.01883-25

    Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

    Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

    Techniques: Activation Assay, Expressing, Infection, Control